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Addgene

pEGFP-N3-hARL1(T31N)
(Plasmid #206408)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206408 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARL1
  • Species
    H. sapiens (human)
  • Mutation
    T31N
  • Entrez Gene
    ARL1 (a.k.a. ARFL1)
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N3-hARL1(T31N) was a gift from Kazuhisa Nakayama & Hye-Won Shin (Addgene plasmid # 206408 ; http://n2t.net/addgene:206408 ; RRID:Addgene_206408)

  • For your References section:

    Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking. Shin HW, Kobayashi H, Kitamura M, Waguri S, Suganuma T, Uchiyama Y, Nakayama K. J Cell Sci. 2005 Sep 1;118(Pt 17):4039-48. doi: 10.1242/jcs.02524. 10.1242/jcs.02524 PubMed 16129887
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