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Addgene

pcDNA3-hARFRP1(T31N)-HA
(Plasmid #206401)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206401 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARFRP1
  • Species
    H. sapiens (human)
  • Mutation
    T31N
  • Entrez Gene
    ARFRP1 (a.k.a. ARL18, ARP, Arp1)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-hARFRP1(T31N)-HA was a gift from Kazuhisa Nakayama & Hye-Won Shin (Addgene plasmid # 206401 ; http://n2t.net/addgene:206401 ; RRID:Addgene_206401)

  • For your References section:

    Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs. Man Z, Kondo Y, Koga H, Umino H, Nakayama K, Shin HW. J Biol Chem. 2011 Apr 1;286(13):11569-78. doi: 10.1074/jbc.M110.201442. Epub 2011 Jan 14. 10.1074/jbc.M110.201442 PubMed 21239483
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