pcDNA3-hARFRP1(Q79L)-HA
(Plasmid
#206400)
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PurposeMammalian expression of hARFRP1(Q79L)
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameARFRP1
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SpeciesH. sapiens (human)
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MutationQ79L
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Entrez GeneARFRP1 (a.k.a. ARL18, ARP, Arp1)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-hARFRP1(Q79L)-HA was a gift from Kazuhisa Nakayama & Hye-Won Shin (Addgene plasmid # 206400 ; http://n2t.net/addgene:206400 ; RRID:Addgene_206400) -
For your References section:
Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs. Man Z, Kondo Y, Koga H, Umino H, Nakayama K, Shin HW. J Biol Chem. 2011 Apr 1;286(13):11569-78. doi: 10.1074/jbc.M110.201442. Epub 2011 Jan 14. 10.1074/jbc.M110.201442 PubMed 21239483