pHAGE2-TetO-ePOUSKM
(Plasmid
#206398)
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PurposeExpresses four genes (human ePOU, SOX2, KLF4, c-MYC) in mammalian cells, for lentivirus generation.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206398 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHAGE2-TetO
- Backbone size w/o insert (bp) 6300
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE2-TetO-ePOUSKM was a gift from Ralf Jauch (Addgene plasmid # 206398 ; http://n2t.net/addgene:206398 ; RRID:Addgene_206398)