pCXLE_hePOU_shp53
(Plasmid
#206397)
-
PurposeIntegration-free (episomal) expression of human ePOU and shRNA against p53.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 206397 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCXLE
- Backbone size w/o insert (bp) 10200
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert nameePOU
-
SpeciesH. sapiens (human)
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
Gene/Insert 2
-
Gene/Insert nameshRNA against p53
-
SpeciesH. sapiens (human)
-
Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCXLE_hePOU_shp53 was a gift from Ralf Jauch (Addgene plasmid # 206397 ; http://n2t.net/addgene:206397 ; RRID:Addgene_206397)