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Addgene

pLVTHM_mePOU_3xFlag
(Plasmid #206395)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206395 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVTHM-3xflag
  • Backbone size w/o insert (bp) 10247
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ePOU
  • Species
    M. musculus (mouse)
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVTHM_mePOU_3xFlag was a gift from Ralf Jauch (Addgene plasmid # 206395 ; http://n2t.net/addgene:206395 ; RRID:Addgene_206395)

  • For your References section:

    Directed Evolution of an Enhanced POU Reprogramming Factor for Cell Fate Engineering. Tan DS, Chen Y, Gao Y, Bednarz A, Wei Y, Malik V, Ho DH, Weng M, Ho SY, Srivastava Y, Velychko S, Yang X, Fan L, Kim J, Graumann J, Stormo GD, Braun T, Yan J, Scholer HR, Jauch R. Mol Biol Evol. 2021 Jun 25;38(7):2854-2868. doi: 10.1093/molbev/msab075. 10.1093/molbev/msab075 PubMed 33720298
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