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pET28 mOct4-POU
(Plasmid #206393)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206393 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Oct4
  • Species
    M. musculus (mouse)
  • Mutation
    POU DNA binding domain only
  • Entrez Gene
    Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28 mOct4-POU was a gift from Ralf Jauch (Addgene plasmid # 206393 ; http://n2t.net/addgene:206393 ; RRID:Addgene_206393)

  • For your References section:

    Directed Evolution of an Enhanced POU Reprogramming Factor for Cell Fate Engineering. Tan DS, Chen Y, Gao Y, Bednarz A, Wei Y, Malik V, Ho DH, Weng M, Ho SY, Srivastava Y, Velychko S, Yang X, Fan L, Kim J, Graumann J, Stormo GD, Braun T, Yan J, Scholer HR, Jauch R. Mol Biol Evol. 2021 Jun 25;38(7):2854-2868. doi: 10.1093/molbev/msab075. 10.1093/molbev/msab075 PubMed 33720298
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