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Addgene

AAV-RO1.7-Hsp90ab1-FLAG
(Plasmid #206355)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206355 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-RO1.7-W3SL
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hsp90ab1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1248
  • Entrez Gene
    Hsp90ab1 (a.k.a. 90kDa, AL022974, C81438, Hsp84, Hsp84-1, Hsp90, Hspcb)
  • Promoter 1.7kb human red opsin
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gggacagggctttccatagcatATGCCTGAGGAAGTGCACCA
  • 3′ sequencing primer tgattatctcgagcggGCTActtgtcgtcatcgtctttgt
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-RO1.7-Hsp90ab1-FLAG was a gift from Connie Cepko (Addgene plasmid # 206355 ; http://n2t.net/addgene:206355 ; RRID:Addgene_206355)

  • For your References section:

    Txnip deletions and missense alleles prolong the survival of cones in a retinitis pigmentosa mouse model. Xue Y, Zhou Y, Cepko CL. Elife. 2024 May 10;12:RP90749. doi: 10.7554/eLife.90749. 10.7554/eLife.90749 PubMed 38727583
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