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Addgene

AAV-RedO-nt.Txnip(C247S)(1-301aa)
(Plasmid #206354)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206354 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-RedO-WPRE-bGH
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Txnip
  • Alt name
    VDUP1
  • Alt name
    ARRDC6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    903
  • Mutation
    (1-301aa), C247S
  • GenBank ID
    NM_001009935.2
  • Entrez Gene
    Txnip (a.k.a. 1200008J08Rik, Hyplip1, THIF, Tbp-2, VDUP1)
  • Promoter human red opsin

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer acagggctttccatagcggtaccATGGTGATGTTCAAGAAGAT
  • 3′ sequencing primer ccagaggttgattggatccactaAGACCTGCTGCCAATCACTA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-RedO-nt.Txnip(C247S)(1-301aa) was a gift from Connie Cepko (Addgene plasmid # 206354 ; http://n2t.net/addgene:206354 ; RRID:Addgene_206354)

  • For your References section:

    Txnip deletions and missense alleles prolong the survival of cones in a retinitis pigmentosa mouse model. Xue Y, Zhou Y, Cepko CL. Elife. 2024 May 10;12:RP90749. doi: 10.7554/eLife.90749. 10.7554/eLife.90749 PubMed 38727583
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