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Addgene

AAV-RO1.7-RPE65
(Plasmid #206346)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206346 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-RO1.7-W3SL
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rpe65
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1602
  • Mutation
    450-Met (C57BL6 natural variation)
  • Entrez Gene
    Rpe65 (a.k.a. 65kDa, A930029L06Rik, LCA2, Mord1, RP20, rd12)
  • Promoter 1.7kb human red opsin

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gacagggctttccatagcAcATGTCTATCCAAATTGAACA
  • 3′ sequencing primer ggttgattatctcgagcggGCTAGGATCTTTTGAACAGTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-RO1.7-RPE65 was a gift from Connie Cepko (Addgene plasmid # 206346 ; http://n2t.net/addgene:206346 ; RRID:Addgene_206346)

  • For your References section:

    Chromophore supply modulates cone function and survival in retinitis pigmentosa mouse models. Xue Y, Sun X, Wang SK, Collin GB, Kefalov VJ, Cepko CL. Proc Natl Acad Sci U S A. 2023 Jun 6;120(23):e2217885120. doi: 10.1073/pnas.2217885120. Epub 2023 May 30. 10.1073/pnas.2217885120 PubMed 37252956
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