AAV-RO1.7-dnHIF
(Plasmid #206343)

• Purpose: AAV plasmid expressing dominant negative HIF1A in photoreceptors
• Depositing Lab: Connie Cepko
• Publication: Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi: 10.7554/eLife.66240.

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Backbone

• Vector backbone: pAAV-RO1.7-W3SL
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dominant negative hif1a
• Species: M. musculus (mouse)
• Insert Size (bp): 1098
• Mutation: dominant negative HIF1A
• Promoter: 1.7kb human red opsin

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gacagggctttccatagcATatgTCTCGGCGAAGCAAAGAG
• 3′ sequencing primer: ggttgattatctcgagcggGtcaCTTAAGCTTATCAAAAAGGCAGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry