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AAV-RO1.7-dnHIF
(Plasmid #206343)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206343 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-RO1.7-W3SL
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dominant negative hif1a
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1098
  • Mutation
    dominant negative HIF1A
  • Promoter 1.7kb human red opsin

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gacagggctttccatagcATatgTCTCGGCGAAGCAAAGAG
  • 3′ sequencing primer ggttgattatctcgagcggGtcaCTTAAGCTTATCAAAAAGGCAGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-RO1.7-dnHIF was a gift from Connie Cepko (Addgene plasmid # 206343 ; http://n2t.net/addgene:206343 ; RRID:Addgene_206343)

  • For your References section:

    AAV-Txnip prolongs cone survival and vision in mouse models of retinitis pigmentosa. Xue Y, Wang SK, Rana P, West ER, Hong CM, Feng H, Wu DM, Cepko CL. Elife. 2021 Apr 13;10. pii: 66240. doi: 10.7554/eLife.66240. 10.7554/eLife.66240 PubMed 33847261
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