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Addgene

pBstSK+IRES-V5-IPpoI (mutant)
(Plasmid #206322)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206322 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 4056
  • Vector type
    mRNA production

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRES element-V5 epitope tag-mutated IPpoI open reading frame
  • Species
    Synthetic
  • Insert Size (bp)
    1131
  • Tag / Fusion Protein
    • V5-IPpoI fusion protein (mutant version) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBstSK+IRES-V5-IPpoI (mutant) was a gift from Brian McStay (Addgene plasmid # 206322 ; http://n2t.net/addgene:206322 ; RRID:Addgene_206322)

  • For your References section:

    A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage. van Sluis M, McStay B. Genes Dev. 2015 Jun 1;29(11):1151-63. doi: 10.1101/gad.260703.115. Epub 2015 May 27. 10.1101/gad.260703.115 PubMed 26019174
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