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Addgene

pUC-hrDNA-12.0
(Plasmid #206320)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206320 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC9
  • Total vector size (bp) 14598
  • Vector type
    cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Intergenic spacer of human rDNA repeat
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    11939

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer m13 fwd
  • 3′ sequencing primer m13 rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: The plasmid contains minor discrepancies in the rDNA repeat region. These discrepancies are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC-hrDNA-12.0 was a gift from Brian McStay (Addgene plasmid # 206320 ; http://n2t.net/addgene:206320 ; RRID:Addgene_206320)

  • For your References section:

    The Relationship Between Human Nucleolar Organizer Regions and Nucleoli, Probed by 3D-ImmunoFISH. van Sluis M, van Vuuren C, McStay B. Methods Mol Biol. 2016;1455:3-14. doi: 10.1007/978-1-4939-3792-9_1. 10.1007/978-1-4939-3792-9_1 PubMed 27576706
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