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pMMK ENTR 2 CMV mKO2-hCdt1
(Plasmid #206282)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206282 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMMK ENTR 2
  • Vector type
    Mammalian Expression ; MultiMate/Gateway ENTR 2

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mKO2-hCdt1
  • Species
    H. sapiens (human)
  • Entrez Gene
    CDT1 (a.k.a. DUP, RIS2)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    mKO2 hCdt1 was obtained through Gibson assembly of PCR amplified p1494 mKO2 hCdt1 (De Jaime-Soguero A. et al. Plos Genetics 2017)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

L224Q and A247S mutations in mKO2-hCdt1 will not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMMK ENTR 2 CMV mKO2-hCdt1 was a gift from Imre Berger (Addgene plasmid # 206282 ; http://n2t.net/addgene:206282 ; RRID:Addgene_206282)

  • For your References section:

    Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus. Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, Welsh GI, Berger I. Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587. 10.1093/nar/gkac587 PubMed 35801912
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