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Addgene

MultiMate-Cell-cycle
(Plasmid #206280)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206280 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMMACE DEST CMV H2B IRFP713
  • Vector type
    Mammalian Expression ; Recombinant baculovirus production

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B iRFP713, mAG-hGeminin, mKO2-hCdt1
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    H2B-iRFP was PCR amplified from pCAG-H2BtdiRFP-IP (Addgene plasmid #47884,Miyanari Y. et al.Nat Struct Mol Biol. 2013)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

L224Q and A247S mutations in mKO2-hCdt1 will not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MultiMate-Cell-cycle was a gift from Imre Berger (Addgene plasmid # 206280 ; http://n2t.net/addgene:206280 ; RRID:Addgene_206280)

  • For your References section:

    Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus. Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, Welsh GI, Berger I. Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587. 10.1093/nar/gkac587 PubMed 35801912
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