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Addgene

pMMACE DEST CMV PE2
(Plasmid #206276)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206276 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMMACE DEST CMV H2B IRFP713
  • Vector type
    Mammalian Expression, CRISPR ; Recombinant baculovirus production, MultiMate/Gateway DEST, CRE Acceptor (MultiBac)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Gentamicin, 25 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PE2
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    PE2 was obtained by restriction digestion from pCMV PE2 (Addgene plasmid #132775, Anzalone et al. Nature 2019)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMMACE DEST CMV PE2 was a gift from Imre Berger (Addgene plasmid # 206276 ; http://n2t.net/addgene:206276 ; RRID:Addgene_206276)

  • For your References section:

    Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus. Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, Welsh GI, Berger I. Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587. 10.1093/nar/gkac587 PubMed 35801912
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