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Addgene

MultiMate-PE3 HEK3
(Plasmid #206273)

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Item Catalog # Description Quantity Price (USD)
Plasmid 206273 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMMACE DEST CMV PE2
  • Vector type
    Mammalian Expression, CRISPR ; Recombinant baculovirus production

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Gentamicin, 25 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
  • Species
    Other

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    PE2 was obtained by restriction digestion from pCMV PE2 (Addgene plasmid #132775, Anzalone et al. Nature 2019) and the HEK3 PegRNA was PCR amplified from pU6-Sp-pegRNA-HEK3_CTT_ins (Addgene plasmid #132778, Anzalone et al. Nature 2019 ). HEK3 nicking sgRNA (Anzalone et al. Nature 2019) was synthesised Twist Bioscience. aeBlue (Liljeruhm J. et al J. Biol. Eng. 2018) was synthesised by Twist Bioscience and VSVG-G was amplified from pMD2.G (Didier Trono lab, Addgene plasmid # 12259)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MultiMate-PE3 HEK3 was a gift from Imre Berger (Addgene plasmid # 206273 ; http://n2t.net/addgene:206273 ; RRID:Addgene_206273)

  • For your References section:

    Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus. Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, Welsh GI, Berger I. Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587. 10.1093/nar/gkac587 PubMed 35801912
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