pMMACE DEST CMV SpCas9
(Plasmid
#206268)
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PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206268 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMMACE DEST CMV H2B IRFP713
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Vector typeMammalian Expression, CRISPR ; Recombinant baculovirus production
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Gentamicin, 25 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpCas9
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SpeciesS. Pyogenes
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySpCas9 was PCR amplified from px459 (Addgene plasmid#48139, Ran et al. Nat Protocol 2013)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMMACE DEST CMV SpCas9 was a gift from Imre Berger (Addgene plasmid # 206268 ; http://n2t.net/addgene:206268 ; RRID:Addgene_206268) -
For your References section:
Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus. Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, Welsh GI, Berger I. Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587. 10.1093/nar/gkac587 PubMed 35801912
Map uploaded by the depositor.
