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Addgene

pMMDS 2 colors
(Plasmid #206265)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206265 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMMDS DEST
  • Vector type
    Mammalian Expression ; CRE-donor

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GST mTagBFP1 (Synthetic), Ctnnb1 (M. musculus)
  • Species
    Other
  • Entrez Gene
    Ctnnb1 (a.k.a. Bfc, Catnb, Mesc)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    mAG beta-catenin was obtained through Gibson assembly of PCR amplified mAG from pL-EF1a mAG-hGeminin (De Jaime-Soguero A. et al. Plos Genetics 2017), and beta-catenin from pL-EF1a beta-catenin SV40 Puro (Aulicino et al. Stem Cell Reports, 2020)Golgi targeting sequence (GTS) mTagBFP was synthesised by Twist Bioscience. polH or p10 cassettes were amplified from MultiBac vectors (Berger I. et al. Nat Biotechnology 2004)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMMDS 2 colors was a gift from Imre Berger (Addgene plasmid # 206265 ; http://n2t.net/addgene:206265 ; RRID:Addgene_206265)

  • For your References section:

    Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus. Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, Welsh GI, Berger I. Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587. 10.1093/nar/gkac587 PubMed 35801912
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