pMMDS DEST
(Plasmid
#206264)
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Purpose(Empty Backbone) DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206264 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIDS
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Backbone manufacturerGeneva Biotech
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Vector typeCRE-donor, MultiMate/Gateway DEST vector (MultiBac)
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byattR1-Ccdb-Chlo-attR2 cassettes for generating DEST vectors were PCR amplified from pInducer-20 (Addgene plasmid #44012, Meerbrey K.L. et al
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
After LR recombiNation, grow only in Pir+ E.Coli
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMMDS DEST was a gift from Imre Berger (Addgene plasmid # 206264 ; http://n2t.net/addgene:206264 ; RRID:Addgene_206264) -
For your References section:
Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus. Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, Welsh GI, Berger I. Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587. 10.1093/nar/gkac587 PubMed 35801912
Map uploaded by the depositor.
