pMMK ENTR 3 CMV mito mCherry
(Plasmid #206256)

• Purpose: ENTR Vector 3 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mitochondrially localised mCherry under the control of CMV promoter.
• Depositing Lab: Imre Berger
• Publication: Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587.

Backbone

• Vector backbone: pMMK ENTR 3
• Vector type: Mammalian Expression ; MultiMate/Gateway ENTR 3

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mito mCherry
• Species: Synthetic
• Tag / Fusion Protein:
  • COX8 mitochondrial tag (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pMMK ENTR 3 CMV mito mCherry.gb
• A portion of this plasmid was derived from a plasmid made by: Mito-mCherry was generated by fusing mCherry from 7TGC (Addgene plasmid#24304) to the COX8 mitochondrial targeting sequence from 5-colours MultiBac vectors (Mansouri et al. 2016)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry