pMMACE DEST CMV H2B iRFP
(Plasmid #206253)

• Purpose: DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
• Depositing Lab: Imre Berger
• Publication: Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587.

Backbone

• Vector backbone: pACEMam
• Backbone manufacturer: Geneva Biotech
• Vector type: Mammalian Expression ; Recombinant baculovirus production, MultiMate/Gateway DEST, CRE Acceptor (MultiBac)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Gentamicin, 25 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H2B iRFP713
• Species: H. sapiens (human)
• Tag / Fusion Protein:
  • iRFP713 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pMMACE DEST CMV H2B iRFP.gb
• A portion of this plasmid was derived from a plasmid made by: H2B-iRFP was PCR amplified from pCAG-H2BtdiRFP-IP (Addgene plasmid#47884,Miyanari Y. et al.Nat Struct Mol Biol. 2013); attR1-Ccdb-Chlo-attR2 cassettes for generating pMMACE DEST were PCR amplified from pInducer-20 (Addgene plasmid #44012, Meerbrey K.L. et al PNAS 2011)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry