MultiMate-Rainbow
(Plasmid #206252)

• Purpose: Expression of 7 fluorescently labelled proteins in mammalian cells. Can be used to generate recombinant baculovirus particles.
• Depositing Lab: Imre Berger
• Publication: Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587.

Backbone

• Vector backbone: pMMACE DEST CMV H2B IRFP713
• Vector type: Mammalian Expression ; Recombinant baculovirus production (Bac-to-Bac)

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin and Spectinomycin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H2B (human); Actin, Tubulin (B. taurus); mito mCherry, GST mTagBFP1 (Synthetic); CyOFP1 (E. quadricolor); Ctnnb1 (mouse)
• Species: Other
• Tag / Fusion Protein:
  • iRFP713 (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • MultiMate-Rainbow.gb
• A portion of this plasmid was derived from a plasmid made by: H2B-iRFP was PCR amplified from pCAG-H2BtdiRFP-IP (Addgene plasmid#47884,Miyanari Y. et al.Nat Struct Mol Biol. 2013)EYFP-Tubulin and mTFP1-Actin were amplified from 5-colours MultiBac vectors (Mansouri et al. Nat Comm 2016)Mito-mCherry was generated by fusing mCherry from 7TGC (Addgene plasmid#24304) to the COX8 mitochondrial targeting sequence from 5-colours MultiBac vectors (Mansouri et al. 2016)CyOFP1 (Chu et al. Nat Biotechnology 2016)fused to the endoplasmic reticulum targeting sequence (ER) was synthesised by Twist Bioscience.mAG beta-catenin was obtained through Gibson assembly of PCR amplified mAG from pL-EF1a mAG-hGeminin (De Jaime-Soguero A. et al. Plos Genetics 2017), and beta-catenin from pL-EF1a beta-catenin SV40 Puro (Aulicino et al. Stem Cell Reports, 2020)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry