pF CAG mc2 IRES hygro
(Plasmid
#206234)
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Purpose(Empty Backbone) For expressing cloned exons as circular RNA.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206234 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepF CAG IRES hygro
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Vector typeMammalian Expression, Lentiviral, Synthetic Biology
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: BamHI (not destroyed), 3' Cloning Site: XbaI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. This plasmid was created by replacing the neoR gene of pF CAG mc2 IRES neo with the hygroR gene from Addgene plasmid ID 98291. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pF CAG mc2 IRES hygro was a gift from Simon Conn & Brett Stringer (Addgene plasmid # 206234 ; http://n2t.net/addgene:206234 ; RRID:Addgene_206234) -
For your References section:
Versatile toolkit for highly-efficient and scarless overexpression of circular RNAs. Stringer BW, Gabryelska M, Marri S, Clark L, Lin H, Gantley L, Liu R, Wilusz JE, Conn VM, Conn SJ. bioRxiv. 2023 Nov 22:2023.11.21.568171. doi: 10.1101/2023.11.21.568171. Preprint. 10.1101/2023.11.21.568171 PubMed 38045421