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Addgene

pF CAG mc2 IRES neo
(Plasmid #206232)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206232 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pF CAG IRES neo
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: BamHI (not destroyed), 3' Cloning Site: XbaI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. This plasmid was created from Addgene plasmid ID 98294 by replacing the luciferase coding sequence with the BamHI-XbaI restriction fragment of the plasmid mc2 neo. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pF CAG mc2 IRES neo was a gift from Simon Conn & Brett Stringer (Addgene plasmid # 206232 ; http://n2t.net/addgene:206232 ; RRID:Addgene_206232)

  • For your References section:

    Versatile toolkit for highly-efficient and scarless overexpression of circular RNAs. Stringer BW, Gabryelska M, Marri S, Clark L, Lin H, Gantley L, Liu R, Wilusz JE, Conn VM, Conn SJ. bioRxiv. 2023 Nov 22:2023.11.21.568171. doi: 10.1101/2023.11.21.568171. Preprint. 10.1101/2023.11.21.568171 PubMed 38045421
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