pRTP801/TA-ODD
(Plasmid
#206212)
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PurposeAn expression vector of hypoxia-responsive transactivator under control of RTP801 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206212 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUGW
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametetracycline-responsive transactivator fused with oxygen-dependent degradation domain
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Alt nameTA-ODD
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer AGGTGGAGAGAGAGACAGAGACAG
- 3′ sequencing primer CATAGCGTAAAAGGAGCAACA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRTP801/TA-ODD was a gift from Masamichi Kamihira (Addgene plasmid # 206212 ; http://n2t.net/addgene:206212 ; RRID:Addgene_206212) -
For your References section:
Hypoxia-responsive expression of vascular endothelial growth factor for induction of angiogenesis in artificial three-dimensional tissues. Masumoto S, Ono A, Ito A, Kawabe Y, Kamihira M. J Biosci Bioeng. 2021 Oct;132(4):399-407. doi: 10.1016/j.jbiosc.2021.06.010. Epub 2021 Aug 4. 10.1016/j.jbiosc.2021.06.010 PubMed 34364783