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pBR BsmAIM Kan + p15A BsaIR Amp
(Plasmid #206208)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206208 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Modified pCR2.1 TOPO (pBR BsmAIM Kan) and p15A origin plasmid (p15A BsaIR Amp)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Both Ampicillin and Kanamycin must be used to ensure selection for bacteria containing both plasmids.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BsamAIM and BsaIR

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please take both plasmid sequences into account when performing sequencing and digest assays.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBR BsmAIM Kan + p15A BsaIR Amp was a gift from Andrew Ellington (Addgene plasmid # 206208 ; http://n2t.net/addgene:206208 ; RRID:Addgene_206208)

  • For your References section:

    Producing molecular biology reagents without purification. Bhadra S, Nguyen V, Torres JA, Kar S, Fadanka S, Gandini C, Akligoh H, Paik I, Maranhao AC, Molloy J, Ellington AD. PLoS One. 2021 Jun 1;16(6):e0252507. doi: 10.1371/journal.pone.0252507. eCollection 2021. 10.1371/journal.pone.0252507 PubMed 34061896
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