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Addgene

pActin-miRFP704nano
(Plasmid #206191)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206191 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miRFP704nano-human beta-actin
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1623
  • Promoter CMV
  • Tag / Fusion Protein
    • miRFP704nano (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pActin-miRFP704nano was a gift from Vladislav Verkhusha (Addgene plasmid # 206191 ; http://n2t.net/addgene:206191 ; RRID:Addgene_206191)

  • For your References section:

    Development of bright red-shifted miRFP704nano using structural analysis of miRFPnano proteins. Oliinyk OS, Pletnev S, Baloban M, Verkhusha VV. Protein Sci. 2023 Jun 22:e4709. doi: 10.1002/pro.4709. 10.1002/pro.4709 PubMed 37347539
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