ACVR1-TAAS-R206H
(Plasmid
#206184)
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Purposein vitro transcription of RNA encoding human ACVR1 with mutations S190A, S192A, and R206H
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206184 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
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Vector typein vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameactivin A receptor type 1
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Alt nameALK2
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SpeciesH. sapiens (human)
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Insert Size (bp)1599
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MutationS190A, S192A, R206H
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Entrez GeneACVR1 (a.k.a. ACTRI, ACVR1A, ACVRLK2, ALK2, FOP, SKR1, TSRI)
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Tag
/ Fusion Protein
- FLAG tag (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ACVR1-TAAS-R206H was a gift from Mary Mullins (Addgene plasmid # 206184 ; http://n2t.net/addgene:206184 ; RRID:Addgene_206184) -
For your References section:
Reduced GS domain serine/threonine requirements of Fibrodysplasia Ossificans Progressiva mutant type I BMP receptor ACVR1 in the zebrafish. Allen RS, Jones WD, Hale M, Warder BN, Shore EM, Mullins MC. J Bone Miner Res. 2023 Jun 17. doi: 10.1002/jbmr.4869. 10.1002/jbmr.4869 PubMed 37329499
Map uploaded by the depositor.
