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Addgene

pcDNA3.1_RFP
(Plasmid #206177)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206177 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RFP
  • Species
    other
  • Insert Size (bp)
    726
  • Mutation
    NA
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1_RFP was a gift from Christopher Goodnow (Addgene plasmid # 206177 ; http://n2t.net/addgene:206177 ; RRID:Addgene_206177)

  • For your References section:

    SAMD9L autoinflammatory or ataxia pancytopenia disease mutations activate cell-autonomous translational repression. Russell AJ, Gray PE, Ziegler JB, Kim YJ, Smith S, Sewell WA, Goodnow CC. Proc Natl Acad Sci U S A. 2021 Aug 24;118(34):e2110190118. doi: 10.1073/pnas.2110190118. 10.1073/pnas.2110190118 PubMed 34417303
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