pcDNA3.1+N-eGFP_SAMD9L_R986C
(Plasmid
#206176)
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Purposemammalian expression of SAMD9L R986C
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206176 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3.1+N-eGFP
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSAMD9L
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SpeciesH. sapiens (human)
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Insert Size (bp)4757
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MutationR986C
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Entrez GeneSAMD9L (a.k.a. UEF1, DRIF2, C7orf6, FLJ39885, KIAA2005)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1+N-eGFP_SAMD9L_R986C was a gift from Christopher Goodnow (Addgene plasmid # 206176 ; http://n2t.net/addgene:206176 ; RRID:Addgene_206176) -
For your References section:
SAMD9L autoinflammatory or ataxia pancytopenia disease mutations activate cell-autonomous translational repression. Russell AJ, Gray PE, Ziegler JB, Kim YJ, Smith S, Sewell WA, Goodnow CC. Proc Natl Acad Sci U S A. 2021 Aug 24;118(34):e2110190118. doi: 10.1073/pnas.2110190118. 10.1073/pnas.2110190118 PubMed 34417303