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pcDNA3.1+N-eGFP_SAMD9L_Asn885fs
(Plasmid #206175)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206175 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1+N-eGFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SAMD9L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4756
  • Mutation
    N885Tfs*6
  • Entrez Gene
    SAMD9L (a.k.a. UEF1, DRIF2, C7orf6, FLJ39885, KIAA2005)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1+N-eGFP_SAMD9L_Asn885fs was a gift from Christopher Goodnow (Addgene plasmid # 206175 ; http://n2t.net/addgene:206175 ; RRID:Addgene_206175)

  • For your References section:

    SAMD9L autoinflammatory or ataxia pancytopenia disease mutations activate cell-autonomous translational repression. Russell AJ, Gray PE, Ziegler JB, Kim YJ, Smith S, Sewell WA, Goodnow CC. Proc Natl Acad Sci U S A. 2021 Aug 24;118(34):e2110190118. doi: 10.1073/pnas.2110190118. 10.1073/pnas.2110190118 PubMed 34417303
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