Lenti-2XPRE-GFP
(Plasmid #206169)

• Purpose: pMVP Gateway destination vector (3rd generation lentiviral vector) containing 2 consensus PGR binding sites upstream of a minimal 2YBTATA promoter
• Depositing Lab: Renee van Amerongen
• Publication: Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov 29;28(1):24. doi: 10.1007/s10911-023-09550-0.

Backbone

• Vector backbone: pMVP/Lenti/Neo-DEST
• Backbone size w/o insert (bp): 8462
• Total vector size (bp): 8304
• Vector type: Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 2X PRE-eGFP
• Species: H. sapiens (human), Synthetic; PRE is human PGR binding site, 2YBTATA is a synthetic minimal promotor, eGFP is a fluorescent protein derived from Jellyfish
• Insert Size (bp): 1466
• Promoter: 2YBTATA

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Attb1 fw
• 3′ sequencing primer: hGH-PA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gateway plasmids used to make this vector: Addgene: #206166, #121730, #121746, #121850

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry