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Addgene

pMuLE (L1-L4) 2X PRE
(Plasmid #206166)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206166 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMuLE ENTR MCS L1-L4
  • Backbone size w/o insert (bp) 2668
  • Total vector size (bp) 2701
  • Vector type
    Gateway cloning entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    2X PRE
  • Species
    H. sapiens (human), Synthetic; PRE is human PGR binding site, 2YBTATA is a synthetic minimal promotor
  • Insert Size (bp)
    85
  • Promoter 2YBTATA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13 fw
  • 3′ sequencing primer M13 rev
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    2xPRE sequences are the same as in Addgene plasmid #11350

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMuLE (L1-L4) 2X PRE was a gift from Renee van Amerongen (Addgene plasmid # 206166 ; http://n2t.net/addgene:206166 ; RRID:Addgene_206166)

  • For your References section:

    A molecular toolbox to study progesterone receptor signaling. Aarts MT, Wagner M, van der Wal T, van Boxtel AL, van Amerongen R. J Mammary Gland Biol Neoplasia. 2023 Nov 29;28(1):24. doi: 10.1007/s10911-023-09550-0. 10.1007/s10911-023-09550-0 PubMed 38019315
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