Lenti-CMV-PRB
(Plasmid #206165)

• Purpose: pMVP Gateway destination vector (3rd generation lentiviral vector) containing human full length PRB gene
• Depositing Lab: Renee van Amerongen
• Publication: Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov 29;28(1):24. doi: 10.1007/s10911-023-09550-0.

Backbone

• Vector backbone: pLenti6.3/PL/V5-DEST
• Backbone size w/o insert (bp): 8131
• Total vector size (bp): 11749
• Modifications to backbone: Added full length PGR gene
• Vector type: Lentiviral ; pMVP Gateway Destination Vector
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Progesteron receptor B
• Alt name: PRB, PGR
• Species: H. sapiens (human)
• Insert Size (bp): 5241
• GenBank ID: NM_000926.4
• Entrez Gene: PGR (a.k.a. NR3C3, PR)
• Promoter: CMV

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-PA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PGR gene is cloned from Addgene plasmid: #89130 Addgene plasmid #206164 was used during gateway cloning.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry