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Addgene

12x PRE TK luc
(Plasmid #206163)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206163 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    same as Addgene #11350
  • Backbone size w/o insert (bp) 4805
  • Total vector size (bp) 6879
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    12X PRE TK luciferase
  • Species
    H. sapiens (human), Synthetic; firefly luciferase, minimal promotor is from HSV TK, PRE is human PGR binding site
  • Insert Size (bp)
    2172
  • Promoter min TK

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    based on Addgene #11350

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    12x PRE TK luc was a gift from Renee van Amerongen (Addgene plasmid # 206163 ; http://n2t.net/addgene:206163 ; RRID:Addgene_206163)

  • For your References section:

    A molecular toolbox to study progesterone receptor signaling. Aarts MT, Wagner M, van der Wal T, van Boxtel AL, van Amerongen R. J Mammary Gland Biol Neoplasia. 2023 Nov 29;28(1):24. doi: 10.1007/s10911-023-09550-0. 10.1007/s10911-023-09550-0 PubMed 38019315
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