8X PRE TK luc
(Plasmid #206161)

• Purpose: Luciferase reporter construct containing 8 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350
• Depositing Lab: Renee van Amerongen
• Publication: Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov 29;28(1):24. doi: 10.1007/s10911-023-09550-0.

Backbone

• Vector backbone: same as Addgene #11350
• Backbone size w/o insert (bp): 4805
• Total vector size (bp): 6871
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 8X PRE TK luciferase
• Species: H. sapiens (human), Synthetic; firefly luciferase, minimal promotor is from HSV TK, PRE is human PGR binding site
• Insert Size (bp): 2066
• Promoter: min TK

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13 forward
• 3′ sequencing primer: LucNrev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: based on Addgene #11350

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry