pBI-BFP-NL1
(Plasmid #206153)

• Purpose: pBI-BFP-NL1
• Depositing Lab: Erika Holzbaur
• Publication: Dou et al J Cell Biol. 2024 Jun 3;223(6):e202307092. doi: 10.1083/jcb.202307092. Epub 2024 Mar 21.

Backbone

• Vector backbone: pBI-CMV2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3800
• Total vector size (bp): 6400
• Modifications to backbone: replacing AcGFP1 with BFP from pTagBFP-N Vector (Evrogen) at EcoRI / XbaI, then removing the piece of MCS carried over with BFP by cutting with AgeI and EcoRI followed by filling in and ligating.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Neuroligin-1
• Species: M. musculus (mouse)
• Insert Size (bp): 2471
• GenBank ID:
• Entrez Gene: Nlgn1 (a.k.a. 6330415N05Rik, NL1, Nlg1, mKIAA1070)
• Promoter: CMV
• Tag / Fusion Protein:
  • Bicistronic BFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI/NheI (unknown if destroyed)
• 3′ cloning site: EcoRV/SmaI (unknown if destroyed)
• 5′ sequencing primer: 5’-CATCAATGGGCGTGGATAGC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCAG-NL1(-) : Addgene 15260

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry