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Addgene

pBI-BFP-NL1
(Plasmid #206153)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206153 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBI-CMV2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3800
  • Total vector size (bp) 6400
  • Modifications to backbone
    replacing AcGFP1 with BFP from pTagBFP-N Vector (Evrogen) at EcoRI / XbaI, then removing the piece of MCS carried over with BFP by cutting with AgeI and EcoRI followed by filling in and ligating.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neuroligin-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2471
  • GenBank ID
  • Entrez Gene
    Nlgn1 (a.k.a. 6330415N05Rik, NL1, Nlg1, mKIAA1070)
  • Promoter CMV
  • Tag / Fusion Protein
    • Bicistronic BFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI/NheI (unknown if destroyed)
  • 3′ cloning site EcoRV/SmaI (unknown if destroyed)
  • 5′ sequencing primer 5’-CATCAATGGGCGTGGATAGC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pCAG-NL1(-) : Addgene 15260

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBI-BFP-NL1 was a gift from Erika Holzbaur (Addgene plasmid # 206153 ; http://n2t.net/addgene:206153 ; RRID:Addgene_206153)

  • For your References section:

    RAB3 phosphorylation by pathogenic LRRK2 impairs trafficking of synaptic vesicle precursors. Dou D, Aiken J, Holzbaur ELF. J Cell Biol. 2024 Jun 3;223(6):e202307092. doi: 10.1083/jcb.202307092. Epub 2024 Mar 21. 10.1083/jcb.202307092 PubMed 38512027
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