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Addgene

CMV HA-MADD
(Plasmid #206150)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206150 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5-HA vector
  • Backbone manufacturer
    Adapted from Addgene# 17613
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 10200
  • Modifications to backbone
    replaced Parkin with MADD
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MADD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5500
  • Entrez Gene
    MADD (a.k.a. DEEAH, DENN, IG20, NEDDISH, RAB3GEP, RabGEF)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (filled-in) / HindIII (filled-in); HindIII restored (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer 5’- ATT TAG GTG ACA CTA TAG AA
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    MADD cDNA from Transomic Technologies

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV HA-MADD was a gift from Erika Holzbaur (Addgene plasmid # 206150 ; http://n2t.net/addgene:206150 ; RRID:Addgene_206150)

  • For your References section:

    RAB3 phosphorylation by pathogenic LRRK2 impairs trafficking of synaptic vesicle precursors. Dou D, Aiken J, Holzbaur ELF. J Cell Biol. 2024 Jun 3;223(6):e202307092. doi: 10.1083/jcb.202307092. Epub 2024 Mar 21. 10.1083/jcb.202307092 PubMed 38512027
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