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Addgene

pIRES-H3.3-G34R-FLAG-BFP
(Plasmid #206140)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206140 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H3.3
  • Species
    H. sapiens (human)
  • Mutation
    G34R
  • Entrez Gene
    H3-3A (a.k.a. BRYLIB1, H3-3B, H3.3A, H3F3, H3F3A)
  • Tag / Fusion Protein
    • FLAG-eBFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES-H3.3-G34R-FLAG-BFP was a gift from Maria Castro (Addgene plasmid # 206140 ; http://n2t.net/addgene:206140 ; RRID:Addgene_206140)
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