pT2/shαSMA/GFP4_Seq1/clone1
(Plasmid
#206138)
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PurposeKnockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206138 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepT2
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namealpha-SMA
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Alt nameACTA2
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SpeciesM. musculus (mouse)
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Entrez GeneActa2 (a.k.a. 0610041G09Rik, Actvs, SMAalpha, SMalphaA, a-SMA, alphaSMA)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT2/shαSMA/GFP4_Seq1/clone1 was a gift from Maria Castro (Addgene plasmid # 206138 ; http://n2t.net/addgene:206138 ; RRID:Addgene_206138)