cacnb1b pcDNA3
(Plasmid
#206105)
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Purposeexpression of rat beta1b calcium channel auxillary subunit
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206105 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen Life Technologies
- Backbone size w/o insert (bp) 5446
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecacnb1b
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Alt namebeta1b
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SpeciesR. norvegicus (rat)
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Entrez GeneCacnb1 (a.k.a. CAB1)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CTGGCTAACTAGAGAACC
- 3′ sequencing primer GCATTTAGGTGACACTATAG (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byTerry P. Snutch, Kevin Campbell: Pragnell et al. 1991 https://pubmed.ncbi.nlm.nih.gov/1657644/
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cacnb1b pcDNA3 was a gift from Annette Dolphin (Addgene plasmid # 206105 ; http://n2t.net/addgene:206105 ; RRID:Addgene_206105)