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Addgene

GFP Cav2.2 W391A pRK5
(Plasmid #206084)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206084 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 4716
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cacna1b
  • Alt name
    Cav2.2 alpha1B
  • Species
    O. cuniculus (rabbit)
  • Insert Size (bp)
    7797
  • Mutation
    W391A
  • Entrez Gene
    CACNA1B
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer TTTGCCTTTCTCTCCACA
  • 3′ sequencing primer TGAGTTTGGACAAACCAC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Yasuo Mori

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' end of Cav2.2 DNA is very GC rich

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP Cav2.2 W391A pRK5 was a gift from Annette Dolphin (Addgene plasmid # 206084 ; http://n2t.net/addgene:206084 ; RRID:Addgene_206084)

  • For your References section:

    Beta-subunits promote the expression of Ca(V)2.2 channels by reducing their proteasomal degradation. Waithe D, Ferron L, Page KM, Chaggar K, Dolphin AC. J Biol Chem. 2011 Mar 18;286(11):9598-611. doi: 10.1074/jbc.M110.195909. Epub 2011 Jan 13. 10.1074/jbc.M110.195909 PubMed 21233207
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