EGFP Rab11a S25N pcDNA3.1 Zeo (+)
(Plasmid
#206080)
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Purposedominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206080 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1 Zeo(+)
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Backbone manufacturerInvitrogen Life Technologies
- Backbone size w/o insert (bp) 5015
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRAB11A
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SpeciesH. sapiens (human)
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MutationS25N
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Entrez GeneRAB11A (a.k.a. YL8)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CTGGCTAACTAGAGAACC
- 3′ sequencing primer GTGTCAGCTCCGACTAG (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byProf. T. Hebert
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP Rab11a S25N pcDNA3.1 Zeo (+) was a gift from Annette Dolphin (Addgene plasmid # 206080 ; http://n2t.net/addgene:206080 ; RRID:Addgene_206080) -
For your References section:
The alpha2delta ligand gabapentin inhibits the Rab11-dependent recycling of the calcium channel subunit alpha2delta-2. Tran-Van-Minh A, Dolphin AC. J Neurosci. 2010 Sep 22;30(38):12856-67. doi: 10.1523/JNEUROSCI.2700-10.2010. 10.1523/JNEUROSCI.2700-10.2010 PubMed 20861389
