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Addgene

FMRP shRNA
(Plasmid #206074)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206074 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pG418-shRNA
  • Backbone manufacturer
    Anne Cahill
  • Backbone size w/o insert (bp) 5086
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FMRP
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Fmr1 (a.k.a. FMRP, Fmr-1)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BpiI (unknown if destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FMRP shRNA was a gift from Annette Dolphin (Addgene plasmid # 206074 ; http://n2t.net/addgene:206074 ; RRID:Addgene_206074)

  • For your References section:

    Fragile X mental retardation protein controls synaptic vesicle exocytosis by modulating N-type calcium channel density. Ferron L, Nieto-Rostro M, Cassidy JS, Dolphin AC. Nat Commun. 2014 Apr 7;5:3628. doi: 10.1038/ncomms4628. 10.1038/ncomms4628 PubMed 24709664
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