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Addgene

GFP hSmad4-HomDonor
(Plasmid #206044)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206044 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PURO-2A-eGFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Smad4
  • Species
    H. sapiens (human)
  • Entrez Gene
    SMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)
  • Tag / Fusion Protein
    • EGFP

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP hSmad4-HomDonor was a gift from Ali Hemmati Brivanlou (Addgene plasmid # 206044 ; http://n2t.net/addgene:206044 ; RRID:Addgene_206044)

  • For your References section:

    Morphogen and community effects determine cell fates in response to BMP4 signaling in human embryonic stem cells. Nemashkalo A, Ruzo A, Heemskerk I, Warmflash A. Development. 2017 Sep 1;144(17):3042-3053. doi: 10.1242/dev.153239. Epub 2017 Jul 31. 10.1242/dev.153239 PubMed 28760810
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