pAAVS1-Puro-CAG-mNG2(1-10)
(Plasmid #206042)

• Purpose: Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.
• Depositing Lab: Alisa Piekny
• Publication: Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7554/eLife.92819.

Backbone

• Vector backbone: pYTK089
• Backbone size w/o insert (bp): 1874
• Total vector size (bp): 7451
• Vector type: Mammalian Expression, CRISPR, Synthetic Biology ; Donor template
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
• Alt name: AAVS1-Puro-CAG-mNG2(1-10)
• Species: H. sapiens (human)
• Insert Size (bp): 5577

Resource Information

• Supplemental Documents:
  • paavs1-puro-cag-mng21-10.gb
• A portion of this plasmid was derived from a plasmid made by: The AAVS1 repair template (except for the mNG2(1-10) gene) was obtained from pAAVS1-P-CAG-GFP (Knut Woltjen, Addgene plasmid 80491). The pYTK089 was obtained from John Dueber (Addgene plasmid # 65196).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.06.28.546942 for bioRxiv preprint.