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Addgene

pCMV-ABCB10-ChR2-eYFP
(Plasmid #206037)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206037 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.0
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ChR2
  • Alt name
    channelrhodopsin 2
  • Species
    Chlamydomonas reinhardtii
  • Tags / Fusion Proteins
    • ABCB10 mitochondrial leading sequence (N terminal on insert)
    • EYFP (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-ABCB10-ChR2-eYFP was a gift from Lufang Zhou (Addgene plasmid # 206037 ; http://n2t.net/addgene:206037 ; RRID:Addgene_206037)

  • For your References section:

    Precisely Control Mitochondria with Light to Manipulate Cell Fate Decision. Ernst P, Xu N, Qu J, Chen H, Goldberg MS, Darley-Usmar V, Zhang JJ, O'Rourke B, Liu X, Zhou L. Biophys J. 2019 Aug 20;117(4):631-645. doi: 10.1016/j.bpj.2019.06.038. Epub 2019 Jul 26. 10.1016/j.bpj.2019.06.038 PubMed 31400914
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