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pET28-hXRCC1pd-HIS
(Plasmid #206036)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206036 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28c(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5206
  • Total vector size (bp) 7141
  • Modifications to backbone
    modified MCS including C-terminal thrombin cleavage site and 6xHIS tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hXRCC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1908
  • Mutation
    S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A
  • Promoter T7
  • Tag / Fusion Protein
    • HIS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-hXRCC1pd-HIS was a gift from Primo Schaer (Addgene plasmid # 206036 ; http://n2t.net/addgene:206036 ; RRID:Addgene_206036)

  • For your References section:

    Covalent PARylation of DNA base excision repair proteins regulates DNA demethylation. Schwarz SD, Xu J, Gunasekera K, Schurmann D, Vagbo CB, Ferrari E, Slupphaug G, Hottiger MO, Schar P, Steinacher R. Nat Commun. 2024 Jan 2;15(1):184. doi: 10.1038/s41467-023-44209-8. 10.1038/s41467-023-44209-8 PubMed 38167803
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