pEF1a-mXRCC1pd-eGFP
(Plasmid #206035)

• Purpose: Mammalian expression of PAR-deficient mXRCC1pd coupled to eGFP under the control of a EF1a promoter
• Depositing Lab: Primo Schaer
• Publication: Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 10.1038/s41467-023-44209-8.

Backbone

• Vector backbone: pTwist-Ef1a
• Backbone manufacturer: Twist Biosciences
• Backbone size w/o insert (bp): 6831
• Total vector size (bp): 9503
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mXRCC1pd
• Species: M. musculus (mouse)
• Insert Size (bp): 2745
• Mutation: S105A, S186A, K188A, S195A, S221A, S222A,S238A, S268A
• Promoter: Ef1a
• Tag / Fusion Protein:
  • eGFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: EBV-rev

Resource Information

• Supplemental Documents:
  • pEF1a-mXRCC1pd_GFP.gb
• A portion of this plasmid was derived from a plasmid made by: Twis Biosciences

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CDS of mXRCC1pd was codon-optimized and synthesized with Twist in the mentioned bkacbone