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pEF1a-mXRCC1pd-eGFP
(Plasmid #206035)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206035 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist-Ef1a
  • Backbone manufacturer
    Twist Biosciences
  • Backbone size w/o insert (bp) 6831
  • Total vector size (bp) 9503
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mXRCC1pd
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2745
  • Mutation
    S105A, S186A, K188A, S195A, S221A, S222A,S238A, S268A
  • Promoter Ef1a
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Twis Biosciences

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CDS of mXRCC1pd was codon-optimized and synthesized with Twist in the mentioned bkacbone

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1a-mXRCC1pd-eGFP was a gift from Primo Schaer (Addgene plasmid # 206035 ; http://n2t.net/addgene:206035 ; RRID:Addgene_206035)

  • For your References section:

    Covalent PARylation of DNA base excision repair proteins regulates DNA demethylation. Schwarz SD, Xu J, Gunasekera K, Schurmann D, Vagbo CB, Ferrari E, Slupphaug G, Hottiger MO, Schar P, Steinacher R. Nat Commun. 2024 Jan 2;15(1):184. doi: 10.1038/s41467-023-44209-8. 10.1038/s41467-023-44209-8 PubMed 38167803
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